ABSTRACT
Mesh-related visceral complications caused by mesh erosion after tension-free inguinal hernia repair are one kind of rare long-term complications, but they are easily neglected. Interval time from initial hernia repair to mesh-related visceral complications by preperitoneal and laparoscopic repair is short. Rutkow and transabdominal preperitoneal repair have the highest reported rate. Lichtenstein has the longest interval time and the lowest reported rate. The most frequently eroded organs are sigmoid colon, bladder and small intestine. The common clinical manifestations of sigmoid colon erosion are hematochezia, abdominal wall fistula and colitis, hematuria and recurrent urinary tract infection in bladder erosion cases, intestinal obstruction and abdominal wall fistula in intestinal erosion case, sigmoid-bladder fistula and intestinal-bladder fistula in multiple organ erosion cases. Resection or repair of corresponding organs with mesh removal have good efficacies in most patients. The authors summarize and analyze researches on mesh-related visceral complications after tension-free inguinal hernia repair from 1994 to 2021, review their advances, in order to raise awareness of such complications in clinicians.
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Objective To explore the phenomenon of gene silence of SLC5A8 in breast cancer.Methods Thirty-two patients suffered with breast cancer,hospitalized from June 2014 to June 2015 in affiliated hospital of Hebei university,were collected with the cancer tissue and normal specimen.Real-time quantitative PCR method was used to detect SLC5A8 gene transcription.Paired t-test and-test were used to anaylsed the results.Results Twenty cases of breast cancer tissues (62.5％) have no SLC5A8 gene transcription product,normal breast tissue adjacent to carcinoma specimens that had no product gene transcription was only five (12.5％).SLC5A8 inactivation rate of cancer tissue was significantly higher than normal tissue adjacent to carcinoma (P ＜ 0.05).The SLC5A8 mRNA transcription level of cancer tissue was significantly lower than the normal (P ＜ 0.05).Conclusion SLC5A8 gene transcription silence is the important events in the process of breast cancer occurrence and development.
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Objective To analyze the transcription level of new tumor suppressor gene SLC5A8 mRNA in colorectal cancer tissues.Methods Collected specimens of 23 cases with colorectal cancer and cut out carcinoma tissues and Pericarcinomatous tissue respectively,then used real time fluorescent quantitative PCR (RT-qPCR)to detect the transcription level of gene SLC5A8 mRNA,and the results of carcinoma tissues and pericarcinomatous tissues were analyzed by t test.Results The transcription level of new tumor suppressor gene SLC5A8 mRNA in colorectal cancer tissues is significantly lower than pericarcinomatous tissue (P =0.002).Conclusion The expression of gene SLC5A8 in colorectal cancer tissues is declining or missing,suggesting it has a certain relationship with the incidence of colorectal cancer.
ABSTRACT
Current research suggests that SLC5A8 is a new candidate tumor-suppressor gene whose expression is reduced or deficient in human thyroid cancer,esophageal cancer,gastric cancer,colon cancer and acute myeloid leukemia.The gene is in human chromosomes 12q13-23,and encoding proteins that belongs to the members of the family of sodium/glucose transporters.It is sodium-coupled carrier that transfer short chain fatty acid,lactic acid,nicotinic acid and iodide.The exon 1 of GPG island methylation and histone acetylation can make SLC5A8 gene express silenced and promote the occurrence of tumor.of all transporter substrates of SLC5A8 expresses,butyrate,propionate and pyruvate function as histone deacetylase inhibitors and can be mediated apoptosis of many kinds of tumor cells,which may be the mechanism of inhibiting tumor.
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Objective To investigate the expression of platelet derived growth factor(PDGF) in small bowel transplantation of rats.Methods Isogeneic and allogeneic small bowel transplantation were performed in rats by microsurgical technology.All rats were divided into two groups:isogeneic control group and allogeneic test group.Transplanted tissues were test on 7th,28th and 90th after surgery.Positioning using immunohistochemical method the expression of PDGF.Real time PCR and immunohistochemical staining were also performed to detect the expression of PDGF.Results The unique feature including intestinal graft fibrosis was showed in tissues.Immunohistochemistry results showed PDGF expression was higher in intestinal glands.PDGF mRNA levels in transplanted tissues showed a gradual upward trend,and the top levels is in POD90.Conclusion PDGF expression was significantly higher in the late of intestinal transplantation,which showed an guideline for chronic rejection of intestinal transplantation.
ABSTRACT
Recently,there was great development in the field of small bowel transplantation,which has been become effective in clinical research.However,similarly with the transplantations of heart,lung,liver and kidney,chronic rejection was becoming an important factor for the long-term survivals of grafts.With the deepening comprehensions towards the mechanism of chronic rejection,more methods and therapies were tried and tested.
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Objective To study the extraction and purification of glycosaminoglycans from Rhoqilema esculenta Kishinouye,optimize the enzymolysis process,and its antihyperlipidemia effects were studied.Methods Extraction of glycosaminoglycans(GAG) from Rhopilema esculenta Kishinouye by hydrolyzation method with papain,subtilisin and trypsin neutral proteinase,ethanol precipitation.The rude product of glycosaminoglycans(GAG) was purified and fractionated by the methods of adsorption,dialysis,ethanol precipitation and CTAB precipitation.The experimental hyperlipidemia mice induced by high fat forage were studied.The total cholesterol(TC) and triglyceride(TG) levels were determined.Results Optimum enzymolysis process: papain neutral proteinase1.5%,subtilisin neutral proteinase1.5%,treated for 5h;The result showed that the levels of TC and TG decreased to 6.83?1.09 mmol?L-1(P